Ensuring Genome Integrity: Analytical Strategies for CRISPR-Based Editing

• Evaluating Genome Integrity Beyond the Edit Site: Identification and verification of candidate off-target editing sites. Strategies for detecting large structural changes like translocations, large deletions, and off-target transgene integration
• Designing the Right Assays: Compare the strengths and limitations of NGS, ddPCR, and other platforms for quantifying editing efficiency and detecting rare off-target events
• Optimizing for Scalability & Standardization: Key considerations for adapting assays for QC, transfer to release testing, and ensuring scalability in clinical applications

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